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File:Analysis-of-Human-Cytomegalovirus-Encoded-SUMO-Targets-and-Temporal-Regulation-of-SUMOylation-of-pone.0103308.g004.jpg

Analysis-of-Human-Cytomegalovirus-Encoded-SUMO-Targets-and-Temporal-Regulation-of-SUMOylation-of-pone.0103308.g004.jpg ‎(707 × 462 pixels, file size: 51 KB, MIME type: image/jpeg)

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English: (A) 293T cells in a 100-mm dish were cotransfected with 5 µg of plasmids expressing myc-PIAS1 and HA-IE1 or HA-UL53, as indicated. At 48 h, total cell lysates were prepared and immunoprecipitated with an anti-myc antibody, followed by immunoblotting with an anti-HA antibody. The levels of HA-IE1, HA-UL53, and myc-PIAS1 in whole cell lysates (WCL) were also shown by immunoblotting. (B) The GST and GST-IE1 proteins purified from bacteria were used in GST pull-down assays. Five micrograms of GST and GST-IE1 proteins were immobilized on glutathione-Sepharose beads and were incubated with in vitro-translated and [35S]-methionine–labeled PIAS1. Input PIAS1 (5%) and the GST pull-down samples were separated by SDS-PAGE and visualized by autoradiography (upper panels). The purified GST and GST-IE1 used in pull-down assays are shown by SDS-PAGE and Coomassie Brilliant Blue staining (lower panel).
Date
Source Image file from Kim E, Kim Y, Kim Y, Lee M, Hayward G, Ahn J (2014). "Analysis of Human Cytomegalovirus-Encoded SUMO Targets and Temporal Regulation of SUMOylation of the Immediate-Early Proteins IE1 and IE2 during Infection". PLOS ONE. DOI:10.1371/journal.pone.0103308. PMID 25050850. PMC: 4106884.
Author Kim E, Kim Y, Kim Y, Lee M, Hayward G, Ahn J
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