as clear and cloudless at the present moment as they were upon the day of their introduction; while twelve similar tubes, prepared at the same time, in precisely the same way, and exposed to ordinary airfare clogged with mycelium, mould, and bacteria.
With regard to calcined air, a similar propagating glass was caused to cover twelve other tubes filled with the same infusion. The "glass" was exhausted and carefully filled with air, which had passed through a red-hot platinum-tube, containing a roll of red-hot platinum-gauze. Tested by the searching beam, the calcined air was found quite free from floating matter. Not a speck has invaded the limpidity of the infusions exposed to it, while twelve similar tubes, placed outside, have fallen into rottenness.
The experiments with calcined air took another form. Six years ago, it was found that, to render the laboratory air free from floating-matter, it was only necessary to permit a platinum-wire heated to whiteness to act upon it for a sufficient time. Shades containing pear-juice, damson-juice, hay and turnip juice, and water of yeast, were freed from their floating matter in this way. The infusions were subsequently boiled, and permitted to remain in contact with the calcined air. They are quite clear to the present hour; while the same infusions, exposed to common air, became mouldy and rotten long ago.
It has been affirmed by other workers on this question, that turnip and hay infusions, rendered slightly alkaline, are particularly prone to exhibit the phenomena of spontaneous generation. This was not found in the present investigation to be the case. Many such infusions have been prepared, and they have continued for months without sensible alteration.
Finally, with regard to infusions wholly withdrawn from air, a group of test-tubes containing different infusions was boiled under a bell-jar filled with filtered air, and from which subsequently the air was removed as far as possible by a good air-pump. They are now as pellucid as they were at the time of their preparation more than two months ago, while a group of corresponding tubes exposed to the laboratory air has all fallen into rottenness.
There is another form of experiment on which great weight has been laid; that of hermetically-sealed tubes. On the 6th of last April, a discussion on the "Germ-Theory of Disease" was opened before the Pathological Society of London. The meeting was attended by many distinguished medical men, some of whom were profoundly influenced by the arguments, and none of whom disputed the facts brought forward against the theory on that occasion. The following important summary of these was given by Dr. Bastian: "With the view of settling these questions, therefore, we may carefully prepare an infusion from some animal tissue, be it muscle, kidney, or liver; we may place it in a flask whose neck is drawn out and narrowed in the blowpipe-flame; we may boil the fluid, seal the vessel during ebullition,
