2,4,5-T See 2,4-D (Method 5001) for Procedure
C8H5Cl3O3
MW: 255.49
METHOD: 5001, Issue 2
CAS: 93-76-5
RTECS: AJ8400000
EVALUATION: FULL
OSHA : 10 mg/m 3 (2,4-D or 2,4,5-T) NIOSH: 10 mg/m 3; Group I Pesticide ACGIH: 10 mg/m 3
PROPERTIES:
Issue 1: 15 February 1984 Issue 2: 15 August 1994
solid; MP 153 C (2,4,5-T); VP not significant
SYNONYMS: 2,4,5-T: (2,4,5-trichlorophenoxy)acetic acid; Esterone 245; Trioxone; Weedone
SAMPLING SAMPLER:
FILTER (glass fiber, binderless)
MEASUREMENT TECHNIQUE:
HPLC, UV DETECTION
ANALYTE:
2,4,5-T anion
DESORPTION:
15 mL CH 3OH; stand 30 min
INJECTION VOLUME:
50 µL
ELUENT:
0.003 M NaClO 4-0.001 M Na 2B40 7
FLOW RATE: 1 to 3 L/min VOL-MIN: -MAX:
15 L @ 10 mg/m 3 200 L
SHIPMENT:
routine
SAMPLE STABILITY:
at least 1 week @ 25 C
FLOW RATE:
1.7 mL/min
BLANKS:
2 to 10 field blanks per set
DETECTOR:
UV @ 289 nm
COLUMN:
stainless steel, 50 cm x 2-mm ID, packed with Zipax SAX (DuPont) ambient temperature; 6900 kPa (1000 psi)
CALIBRATION:
solutions of analyte in methanol
RANGE:
0.15 to 2 mg per filter
ACCURACY RANGE STUDIED:
5 to 20 mg/m 3 [1,2] (100-L samples)
BIAS:
4.78%
ESTIMATED LOD: 0.030 mg per filter [2]
ˆ rT): 0.053 (2,4,5-T) [2] OVERALL PRECISION (S ACCURACY:
± 14.2%
PRECISION (Sr):
0.025 [2]
APPLICABILITY: This method determines 2,4-D, 2,4,5-T, and their salts, but not their esters. The working range is 1.5 to 20 mg/m 3 of either compound for a 100-L air sample.
INTERFERENCES: High concentrations of esters of either compound do not interfere but require the use of a pre-column to prevent degradation of the HPLC column.
OTHER METHODS: This method combines and replaces Methods S279 [3] and S303 [3] which are the same except for eluent composition and UV detector wavelength.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94