Page:Bergey's manual of determinative bacteriology.djvu/330

Ammonium chloride and the 19 amino acids which were tested may serve as sources of nitrogen; the amino acids may also be utilized as carbon sources (Campbell and Williams, op. cit., 1951a, 506). Nitrites not produced from nitrates. Trimethylamine not produced from tri- methylamine oxide, betaine, choline or acetyl choline (Campbell and Williams, Jour. Bact., 62, 1951a, 250). Inorganic sulfur may serve as a source of sulfur (Campbell and Williams, loc. cit.). Aerobic, facultative. Optimum temperature, 25° C. Habitat: Milk. 13. Achromobacter eurydice (White, 1912) Bergey et al., 1925. {Bacterium eury- dice White, U. S. Dept. of Agr., Bur. of Entomol., Circ. 157, 1912, 3, and U. S. Dept. of Agr. Bull. 810, 1920, 15; Bergey et al., Manual, 2nd ed., 1925, 170; Acineto- bacter eurydice Brisou and Pr^vot, Ann. Inst. Past., 86, 1954, 727.) eu.ry'di.ce. Gr. fem.n. Eurydice the wife of Orpheus. Small, slender rods with slightly rounded ends; occur singly and in pairs. Non-motile. Gram-negative. Gelatin stab : A bluish gray growth occurs along the line of inoculation. No liquefac- tion. Glucose agar colonies: Bluish gray, cir- cular, smooth glistening, entire. Broth: Uniform turbidity with viscid sediment. Potato: Slight, grayish growth. Litmus milk: Unchanged. Acid from glucose, but little or no action on other carbohydrates. Of 19 amino acids tested, none was re- quired for growth; preformed growth fac- tors also were not required (Campbell and Williams, Food Research, 16, 1951a, 506). Ammonium chloride and the 19 amino acids which were tested may serve as sources of nitrogen; the amino acids may also be utilized as carbon sources (Campbell and Williams, loc. cit.). Trimethylamine not produced from tri- methylamine oxide, betaine, choline or acetyl choline (Campbell and Williams, Jour. Bact., 6.?, 1951b, 250). Inorganic sulfur may serve as a source of sulfur (Campbell and Williams, op. cit., 1951a, 506). Aerobic, facultative. Innocuous when fed to bees. Not patho- genic when inoculated subcutaneously in rabbits. Source: Occurs as a secondary invader in European foulbrood of bees. Habitat: Unknown. 14. Achromobacter delmarvae Smart, 1932. (Jour. Bact., 23, 1932, 41; also see Jour. Agr. Research, 51, 1935, 363; Acineto- bacter delmarvae Brisou and Prdvot, Ann. Inst. Past., 86, 1954, 727.) del.mar'vae. M.L. gen. noun delmarvae of Delmarva; named for Delmarva, the name given to an area comprising portions of the states of Delaware, Maryland and Virginia. Short rods which average 0.75 by 1.5 microns; rounded ends; occur singly, in pairs and in short chains. Non-motile. Gram-negative. Gelatin colonies: Similar to agar colonies. Gelatin stab: Scant growth. No lique- faction. Beef -infusion agar colonies: Small, cir- cular, raised, glistening, translucent, blu- ish white, amorphous; margin entire; edges smooth. Agar stab: Abundant growth. Surface growth round, smooth, glistening, bluish white, raised. Filiform growth the whole length of stab, but growth best at top. Agar slant: Abundant, filiform, raised, glistening, smooth, translucent, bluish white growth; no odor; old cultures slightly viscid. Medium unchanged. Nutrient broth: Turbid. Delicate, white pellicle. Sediment abundant, white, slightly stringy. No odor. Color of medium un- changed. Sterile milk: Slow growth. No peptoniza- tion. Coagulation in 12 to 14 days. Milk turns chocolate-brown beginning at top. Litmus milk: Acid, with reduction of litmus in 5 days. Coagulation, with return of pink color in 12 to 14 days. Browning of medium. Potato: Abundant, grayish white, glisten- ing, smooth, raised growth. Medium changes from white to smoke-gray.