the exterior; the latter alone being in some cases penetrated by the fissures. In many of the spheres the line of separation between the outer and inner parts was tolerably well delined. The outer parts were of exactly the same very pale purple tint, as that of the last- formed smaller spheres; and these latter did not include any darker central core.
From these several facts we may conclude that, when vigorous dark-coloured leaves are subjected to the action of carbonate of ammonia, the fluid within the cells of the tentacles often aggregates exteriorly into coherent viscid matter, forming a kind of bag. Small spheres sometimes appear within this bag, and the whole generally soon divides into two or more spheres, which repeatedly coalesce and redivide. After a longer or shorter time the granules in the colourless layer of protoplasm, which flows round the walls, are drawn to and unite with the larger spheres, or form small independent spheres; those latter being of a much paler colour, and more brittle than the first aggregated masses. After the granules of protoplasm have been thus attracted, the layer of flowing protoplasm can no longer be distinguished, though a current of limpid fluid still flows round the walls.
If a leaf is immersed in a very strong, almost concentrated, solution of carbonate of ammonia, the glands are instantly blackened, and they secrete copiously; but no movement of the tentacles ensues. Two leaves thus treated became after 1 hr. flaccid, and seem killed; all the cells in their tentacles contained spheres of protoplasm, but these were small and discoloured. Two other eaves were placed in a solution not quite so strong, and there was well-marked aggregation in 30 m. After 24 hrs. the spherical or more commonly oblong masses of protoplasm became opaque and granular, instead of being as usual translucent: and in the lower cells there were only innumerable minute spherical granules. It was evident that the strength of the solution had interfered with the completion of the process, as we shall see likewise follows from too great heat.
All the foregoing observations relate to the exterior tentacles, which are of a purple colour; but the green pedicels of the short central tentacles are acted on by the carbonate, and by an infusion of raw meat, in exactly the same manner, with the sole difference that the aggregate masses are of a greenish colour; so that the process is in no way dependent on the colour of the fluid within the cells.
Finally, the most remarkable fact with respect to this salt is the extraordinary small amount which suffices to cause aggregation. Full details will be given in the seventh chapter, and here it will be enough to say that with a sensitive leaf the absorption by a gland of of a grain (.000482 mgr.) is enough to cause in the course of one hour well-marked aggregation in the cells immediately beneath the gland.
The Effects of certain other Salts and Fluids.— Two leaves were placed in a solution of one part of acetate of ammonia to about
146 of water, and were acted on quite as energetically, but perhaps
