Milk: Alkaline; clears.
Nitrites produced from nitrates.
Indole produced.
Hydrogen sulfide not produced.
Acid but no gas from glucose, galactose and glycerol. No acid from lactose, maltose or sucrose.
Starch: Feeble hydrolysis.
Grows in 3 per cent salt.
Temperature relations: Optimum between 31° and 34° C. Maximum, 42° C.
Aerobic.
Source: Isolated from brown stripe of Italian millet.
Habitat: Pathogenic on Italian millet, Setaria italica.
100. Pseudomonas tolaasii Paine, 1919. (Ann. Appl. Biol., 5, 1919, 210.)
to.laa'si.i. Tolaas patronymic; tolaasii of Tolaas.
Rods 0.4 to 0.5 by 0.9 to 1.7 microns. Motile with 1 to 5 polar flagella. Gram-negative.
Green fluorescent pigment produced in culture.
Gelatin: Liquefied.
Bouillon agar: Streak develops in 24 hours, dirty bluish white, wet-shining and slightly raised.
Broth: Turbid in 24 hours. Pellicle.
Milk: Becomes alkaline and clears.
Nitrites not produced from nitrates.
Indole production slight.
Acid but no gas from glucose. No acid from lactose or sucrose.
Starch hydrolysis feeble.
Optimum temperature, 25° C.
Source: Isolated in England from brown-spot of cultivated mushrooms.
Habitat: Pathogenic on cultivated mushrooms.
101. Pseudomonas washingtoniae (Pine 1943) Elliott 1951. (Phytomonas washingtoniae Pine, Phytopath., 33, 1943, 1203; Elliott, Man. Bact. Plant Path., 2nd ed., 1951, 100.)
wash.ing.to'ni.ae. M.L. fem.noun Washingtonia a generic name; washingtoniae of Washingtonia.
Rods, 0.69 l)y 1.61 microns, occurring singly or in short chains. Motile with 1 to 3 polar flagella. Gram-negative.
Green pigment in certain media.
Gelatin: Liquefied.
Potato dextrose agar colonies: Circular, smooth, convex, glistening, white to cream, butyrous, edges entire.
Milk: No curd; peptonization with a green color in 7 days.
Indole not produced.
Nitrites not produced from nitrates.
Hydrogen sulfide produced in minute amounts.
Acid but no gas from glucose, fructose and L-arabinose in 24 hours; from galactose and xylose in 48 hours. No acid from sucrose, lactose, cellobiose, maltose, mannitol, D-sorbitol, glycerol, salicin or raffinose.
Starch not hydrolyzed.
Aerobic.
Thermal death point between 47° and 48° C.
Source: Isolated from spots on the leaves of the palm, Washingtonia filifera.
Habitat: Pathogenic on the Washington palm.
102. Pseudomonas barkeri (Berridge, 1924) Clara, 1934. (Bacillus of pear blossom disease. Barker and Grove, Ann. Appl. Biol., 1, 1914, 94; Barker and Grove's organism, Doidge, Ann. Appl. Biol., 4, 1917, 50; B. barkeri Berridge, Ann. Appl. Biol., 11, 1924, 73; Clara, Science, 75, 1934, 11.)
bar'ker.i. M.L. gen.noun barkeri of Barker; named for B. T. P. Barker, one of the two men who first described this organism.
Description from Doidge (op. cit., 1917, 50).
Rods 0.5 to 0.8 by 2.0 to 4.0 microns. Motile with 1 to 4 polar flagella. Gram-negative (Burkholder), not Gram-positive.
Green fluorescent pigment produced in culture.
Gelatin: Liquefied.
Agar: Growth is white, feeble, flat, glistening, smooth-edged.
Broth: Slightly turbid in 24 hours.
Milk: Slowly cleared.
Nitrites not produced from nitrates.
Indole not produced unless culture warmed.
