Page:Bergey's manual of determinative bacteriology.djvu/346

Methylene blue reduced. Temperature relations: Optimum, be- tween 20° and 28° C. Minimum, between 5° and 10° C. Maximum, less than 36° C. Comment: By subsequent designation, Breed (Riassunti delle Comunicazioni, VI Cong. Internaz. di Microbiol., Roma, 1, 1953, 13) selected this species as the type for the genus Agarhacterium Angst. It was felt that this species could be reisolated and reidentified more readily than could the other species placed in this genus by Angst. Source: Isolated from the fronds of Porphyra perforata. Habitat: Found on marine algae. 2. Agarbacterium rhodomelae (Lund- estad, 1928) Breed, comb. nov. {Bacterium rhodomelae Lundestad, Cent. f. Bakt., II Abt., 75, 1928, 331.) rho.do'me.lae. M.L. noun Rhodomela a genus of red algae; M.L. gen. noun rhodome- lae of Rhodomela. Rods, 0.5 to 0.8 by 1.2 to 2.0 microns, with rounded ends, occurring singly, in pairs and, at times, in short chains. Motile. Gram- negative. Fish gelatin colonies: Circular, slightly glistening, opaque, white. Fish gelatin stab: Rapid, infundibuliform liquefaction. Seaweed agar colonies : Circular, flat, thin, transparent, glistening, entire. Agar is dis- solved. Glucose agar slant: Growth moderate, white, becoming orange-yellow, flat, opaque, glistening; undulate margin. Broth: Turbid; pellicle; grayish yellow, slimy sediment. Indole not produced. Carbohydrates not attacked. Starch very slightly hydrol3'zed. Nitrites not produced from nitrates. Aerobic, facultatively anaerobic. Temperature relations: Optimum, be- between 20° and 25° C. Minimum, 0° C. Maximum, between 30° and 32° C. Source : Isolated from water from the Nor- wegian Coast. Habitat: Sea water. 3. Agarbacterii mesentericuin Angst, 1929. {Agarbacterium mesentericus (sic) Angst, Puget Sound Biol. Sta. Pub., 7, 1929, 52.) me.sen.te'ri.cum. Gr. neut.n. mesenteri- um the mesentery; M.L. adj. mesentericus like the mesentery. Short rods, 0.6 by 0.8 micron, with rounded ends, occurring singly and in pairs. No encapsulated. Motile. Gram-negative. All media contained a decoction made by boiling or autoclaving fronds of Iridaea, a seaweed, in sea water or a 3 per cent salt solution. Fish gelatin colonies: Circular, sunken, granular, irregular, crateriform; growth is rapid. Fish gelatin slant: Abundant, spreading, fiat, glistening, smooth, translucent, white, butyrous growth. Liquefaction. Fish gelatin stab : Infundibuliform lique- faction; growth best at top. Agar colonies: Circular, concentrically ringed, flat, entire, granular; growth is moderate. Agar slant: Abundant, filiform, raised, glistening, finely wrinkled when old or dry, opaque, buff, membranous growth. Fish agar slant: Abundant, filiform, flat, glistening, concentrically ringed, opaque, white, butyrous growth. Meat agar slant: Abundant, spreading, flat, glistening, smooth, opaque, white, butyrous growth. Broth: Moderately turbid; no sediment; membranous pellicle. Plain milk unchanged. Potato: Abundant, spreading, raised, glistening, wrinkled, buff to j'ellowish, membranous growth; the potato becomes grayed. Indole not produced. Hydrogen sulfide not produced. Acid from mannitol. No acid from glucose, lactose, xylose, rhamnose or arabinose. Starch is hydrolyzed. Agar is digested; cellulose not attacked. Nitrites not produced from nitrates. Aerobic. Methjdene blue reduced. Temperature relations: Optimum, be- tween 20° and 28° C. Minimum, less than 5° C. Maximum, between 36° and 45° C.