AEROBIC BACTERIA by GC-FAME 0801
METHOD: 0801, Issue 1
EVALUATION: N/A
Issue 1: 15 January 1998
PROPERTIES: viable and culturable, requires oxygen SYNONYMS: Gram positive (+) bacteria, gram negative (−) bacteria, Mycobacterium species (sp) SAMPLING SAMPLER: ANDERSEN IMPACTOR (15 × 100-mm culture plates containing TSBA media)
MEASUREMENT TECHNIQUE:
GAS CHROMATOGRAPHY, FID
ANALYTE:
fatty acid methyl esters (FAME) of aerobic bacteria or Mycobacterium sp
DESORPTION:
1 mL hexane/MTBE (1:1)
INJECTION VOLUME:
2 µL
FLOW RATE: 28.3 L/min [1] VOL-MIN: -MAX:
50 L 300 L
SHIPMENT: keep cold, ship overnight. SAMPLE STABILITY: transfer bacteria to fresh culture media weekly
TEMPERATURE-INJECTION: 250 °C -DETECTOR: 300 °C -COLUMN: 170 °C to 270 °C (5 °C/min)
BLANKS:
CARRIER GAS:
helium, 2.4 mL/min
COLUMN:
capillary, fused silica, 25 m × 0.20-mm ID, 0.33-µm film, Ultra-2 [2]
CALIBRATION:
MIDI fatty acid methyl ester calibration mix (containing various C9–C20 fatty acids)
not applicable ACCURACY
RANGE STUDIED:
not applicable
BIAS:
not applicable
OVERALL PRECISION ( ): not applicable IDENTIFICATION: comparison with profile library ACCURACY:
not applicable
ACCEPTABLE GENUS IDENTIFICATION: similarity index (SI) > 0.30 ACCEPTABLE SPECIES IDENTIFICATION: SI > 0.50
APPLICABILITY: This method is applicable to all viable and culturable bacteria containing C9–C20 fatty acids. The method is applicable to bulk solid and liquid samples containing culturable bacteria, as well as air samples. INTERFERENCES: No specific interferences were identified. However, any fungi, yeasts, or other source of fatty acid materials will affect identification of bacteria. Additionally, any organic contaminants will interfere with the identification process. OTHER METHODS: Method 0800, Bioaerosol Sampling, is a general procedure for sampling bioaerosols in air.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition
