ISOPHORONE
C9H14 O
MW: 138.21
METHOD: 2508, Issue 2
CAS: 78-59-1
2508
RTECS: GW7700000
EVALUATION: FULL
OSHA : 25 ppm NIOSH: 4 ppm; Group III Pesticide ACGIH: C 5 ppm (1 ppm = 5.65 mg/m 3 @ NTP)
PROPERTIES:
Issue 1: 15 May 1984 Issue 2: 15 August 1994
liquid; d 0.923 g/mL @ 25 °C; BP 213 °C; VP 26 kPa (0.2 mm Hg; 260 ppm) @ 20 °C; explosive range 0.8 to 3.8% v/v in air
SYNONYMS: 3,5,5-trimethyl-2-cyclohexen-1-one
SAMPLING SAMPLER:
MEASUREMENT
SOLID SORBENT TUBE (petroleum-based charcoal, 100 mg/50 mg)
TECHNIQUE:
GAS CHROMATOGRAPHY, FID
ANALYTE:
isophorone
FLOW RATE:
0.01 to 1 L/min
EXTRACTION:
1 mL CS 2; stand 30 min
VOL-MIN: -MAX:
2 L @ 25 ppm 25 L
INJECTION VOLUME:
SHIPMENT:
routine
TEMPERATURE-INJECTOR: -DETECTOR: -COLUMN:
SAMPLE STABILITY:
at least 7 days @ 25 °C
CARRIER GAS:
N 2 or He, 30 mL/min
BLANKS:
2 to 10 field blanks per set
COLUMN:
4 m x 3-mm glass, 10% SP2100/0.1% Carbowax 1500 on 100/120 Supelcoport or equivalent
CALIBRATION:
isophorone in CS 2
RANGE:
0.2 to 10 mg per sample [2]
ACCURACY RANGE STUDIED:
67 to 283 mg/m 3 [1] (180-L samples)
ACCURACY:
± 15.3%
BIAS:
5.0%
ˆ rT): OVERALL PRECISION (S
5 µL 200 °C 250 °C 160 °C
ESTIMATED LOD: 0.02 mg per sample PRECISION (Sr):
0.033 [1,2]
0.059 [1]
APPLICABILITY: The working range for this method is 0.35 to 70 ppm (2 to 400 mg/m 3) for a 12-L air sample. High humidity will greatly decrease breakthrough volume.
INTERFERENCES: None identified. Alternate columns may be used, e.g., 10% SP-1000 or DB-2 fused silica capillary column.
OTHER METHODS: This is Method S367 with a different column recommended [2].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
