NICOTINE
C10H14N2
MW: 162.26
METHOD: 2544, Issue 1
CAS: 54-11-5
2544
RTECS: QS5250000
EVALUATION: PARTIAL
OSHA : 0.5 mg/m 3 (skin) NIOSH: 0.5 mg/m 3 (skin); Group I Pesticide ACGIH: 0.5 mg/m 3 (skin)
PROPERTIES:
Issue 1: 15 August 1994
liquid, BP 245.5 °C; density 1.009 g/mL (20 °C)
SYNONYMS: 3-(1-methyl-2-pyrrolidinyl) pyridine
SAMPLING
MEASUREMENT
SAMPLER:
SOLID SORBENT TUBE XAD-2 (100 mg/50 mg)
TECHNIQUE: GAS CHROMATOGRAPHY-NITROGEN PHOSPHOROUS DETECTOR
FLOW RATE:
1.0 L/min
ANALYTE:
nicotine
VOL-MIN: -MAX:
60 L @ 0.5 mg/m 3 400 L
DESORPTION:
1 mL ethyl acetate, stand 30 min
SHIPMENT:
routine
INJECTION VOLUME:
2.0 µL
SAMPLE STABILITY:
at least 5 days at 25 °C [1]
TEMPERATURE-INJECTION: -DETECTOR: -COLUMN:
BLANKS:
2 to 10 field blanks per set
CARRIER GASES:
ACCURACY 3
RANGE STUDIED:
0.3 to 1.2 mg/m [1] (100-L samples)
BIAS:
not determined [2]
ˆ rT): 0.067 [2] OVERALL PRECISION (S ACCURACY:
235 °C 260 °C 165 °C
He, 25 mL/min; fuel, H 2
COLUMN:
glass, 2 m x 2-mm ID, packed with 3% OV-101 on 100/120 mesh Supelcoport
CALIBRATION:
solutions of nicotine in ethyl acetate
RANGE:
0.03 - 0.13 mg per sample
ESTIMATED LOD: not determined [2] PRECISION (Sr):
0.015 [1]
±12%
APPLICABILITY: The working range is 0.3 to 2 mg/m 3 for a 100-L air sample. The probable useful range of this method is likely to extend to lower concentrations than the analytical range specified above. Desorption efficiency must be determined ov er the range evaluated.
INTERFERENCES: None identified.
OTHER METHODS: This is method S293 [2] in a revised format.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
