NICOTINE: METHOD 2551, Issue 1, dated 15 January 1998 - Page 4 of 4 The maximum sampling volume of 600 L was calculated based on the XAD-4 sampling tube capacity of 300 µg nicotine [7] and the NIOSH REL of 0.5mg/m3. Concentrations of environmental tobacco smoke (ETS) typically range from 1 to 100 µg/m3 [1]. At the highest ETS concentration and a sampling rate of 1 L/min, over 3000 L of air would need to be sampled to reach the sampling tube capacity. Sampling rates and times typically employed in field studies would almost never exceed the capacity of the XAD-4 sampling tube. When sampling for nicotine in ETS, only the XAD-4sampling tube is employed. However, when sampling for nicotine where particulate matter is present, such as in the greenhouse study, it may be necessary to use a glass fiber filter (GFF) in series with the XAD-4 sorbent tube. When a GFF is used, it also should be analyzed for nicotine as studies have indicated that nicotine can be adsorbed on particulate matter.
REFERENCES: [1] [2]
[3]
[4] [5] [6] [7]
ASTM [1990]. ASTM standard test method for nicotine in indoor air, Method D 5075-90a, ASTM Committee D-22, November 1990. NIOSH [1994]. Nicotine: Method 2544. In: Eller PM, Cassinelli ME, eds. NIOSH manual of analytical methods, 4th ed., Cincinnati, OH: National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 94-113. EPA [1988]. Determination of nicotine in indoor air using XAD-4 for active sampling and treated filter cassettes for active and passive sampling with gas chromatographic and nitrogen-selective detection. Compendium Method IA-02, Design and Reports Branch, Environmental Monitoring Systems Laboratory, U.S. EPA, Research Triangle Park, N.C., May 1988. Pendergrass SM [1997]. Backup data report for nicotine (unpublished report). NIOSH/DPSE. NIOSH [1977]. Nicotine: Method S293. In: Taylor DG, ed. NIOSH manual of analytical methods, 2nd ed., V. 3, DHHS NIOSH) Publication No. 77-157-C. The Merck Index, 12 ed. [1996]. Nicotine, Quinoline. Whitehouse Station, NJ: Merck and Co. OSHA [1995]. Draft Method for Nicotine. Salt Lake City, UT: OSHA Salt Lake Technical Center.
METHOD WRITTEN BY: Stephanie M. Pendergrass and Larry B. Jaycox, Ph.D. , NIOSH/DPSE
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition
