2,4-D
C8H6Cl2O3
MW: 221.04
METHOD: 5001, Issue 2
CAS: 94-75-7
Issue 1: 15 February 1984 Issue 2: 15 August 1994
PROPERTIES: solid; MP 138 °C; VP not significant
(2,4-dichlorophenoxy)acetic acid; hedonal; Trinoxol
SAMPLING SAMPLER:
RTECS: AG6825000
EVALUATION: FULL
OSHA : 10 mg/m 3 NIOSH: 10 mg/m 3 ACGIH: 10 mg/m 3
SYNONYMS: 2,4-D:
5001
FILTER (glass fiber, binderless)
MEASUREMENT TECHNIQUE:
HPLC, UV DETECTION
ANALYTE:
2,4-D anion
DESORPTION:
15 mL CH 3OH; stand 30 min
INJECTION VOLUME:
50 µL
ELUENT:
0.001 M NaClO 4-0.001 M Na 2B40 7
FLOW RATE: 1 to 3 L/min VOL-MIN: -MAX:
15 L @ 10 mg/m 3 200 L
SHIPMENT:
routine
SAMPLE STABILITY:
at least 1 week @ 25 °C
FLOW RATE:
1.7 mL/min
BLANKS:
2 to 10 field blanks per set
DETECTOR:
UV @ 284 nm
COLUMN:
stainless steel, 50 cm x 2-mm ID, packed with Zipax SAX (DuPont) ambient temperature; 6900 kPa (1000 psi)
CALIBRATION:
solutions of analyte in methanol
RANGE:
0.15 to 2 mg per filter
ACCURACY RANGE STUDIED: BIAS:
5 to 20 mg/m 3 [1,2] (100-L samples) - 1.26%
ESTIMATED LOD: 0.015 mg per filter [1]
ˆ ): 0.051 (2,4-D) [1] OVERALL PRECISION (S rT ACCURACY:
± 10.21%
PRECISION (Sr):
0.01 [1]
APPLICABILITY: This method determines 2,4-D, 2,4,5-T, and their salts, but not their esters. The working range is 1.5 to 20 mg/m 3 of either compound for a 100-L air sample.
INTERFERENCES: High concentrations of esters of either compound do not interfere but require the use of a pre-column to prevent degradation of the HPLC column.
OTHER METHODS: This method combines and replaces Methods S279 [3] and S303 [3] which are the same except for eluent composition and UV detector wavelength.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
