ESTROGENIC COMPOUNDS
FORMULAE: Table 1
MW: Table 1
METHOD: 5044, Issue 1
CAS: Table 2
RTECS: Table 2
EVALUATION: PARTIAL
PROPERTIES:
OSHA: Table 2 NIOSH: Table 2 ACGIH: Table 2 Synonyms:
5044
Issue 1: 15 March 2003
Table 1
Table 2
SAMPLING
MEASUREMENT
SAMPLER:
FILTER, PTFE (37-mm, 2-:m)
TECHNIQUE:
HPLC, UV detection
FLOW RATE:
1 L/min
ANALYTE:
See Table 1
VOL.-MIN: -MAX:
150 L 1000L
EXTRACTION:
Methanol, extraction overnight at room temperature
SHIPMENT:
Ship at ambient temperature.
INJECTION VOLUME:
SAMPLE STABILITY:
30 days at 25 °C [1]
BLANKS:
2 to 10 field blanks per set
ACCURACY RANGE STUDIED:
Not determined
BIAS:
Not determined
OVERALL PRECISION(S r T ):
Not determined
ACCURACY:
Not determined
25 :L
MOBILE PHASE:
60% acetonitrile / 40% water @ 26 °C, 1.75 mL/min
COLUMN:
C18 reversed phase, 150 x 4.6-mm, 5-:m; in-line pre-filter, 2.0- :m
DETECTOR:
UV/Vis at 200 nm and 237 m:
CALIBRATION:
Standards in methanol
RANGE:
$-Estradiol 0.3 to 44 :g/sample Estrone 0.2 to 64 :g/sample Progesterone 0.5 to 64 :g/sample $-Estradiol 3-Benzoate 0.5 to 64 :g/sample [1]
ESTIMATED LOD: Table 3 PRECISION (S r ):
$-Estradiol 0.040 Estrone 0.039 Progesterone 0.030 $-Estradiol 3-Benzoate 0.032 @ 0.5 - 64 :g/sample [1]
APPLICABILITY: This method has not been used to evaluate field air samples. The method is based on a preliminary investigation analyzing field wipe samples collected during a health hazard evaluation at a facility producing birth control pills [1]. Appendix 1 contains information on recovery of analytes from wipe samples. INTERFERENCES: Any compound that elutes at the same HPLC retention times may interfere. OTHER METHODS: No validated methods were found.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition
