ELEMENTS in blood or tissue
M.W.: Table 1
8005
FORMULA: Table 1 CAS: Table 1
RTECS: Table 1
EVALUATION: PARTIAL
Issue 1: 15 May 1985 Issue 2: 15 August 1994
METHOD: 8005, Issue 2
BIOLOGICAL INDICTOR OF: exposure to the following elements or their compounds: antimony, cadmium, chromium, cobalt, copper, iron, lanthanum, lead, lithium, magnesium, manganese, molybdenum, nickel, platinum, silver, strontium, thallium, vanadium, zinc and zirconium.
SYNONYMS: vary according to compound.
BIOLOGICAL SAMPLING SPECIMEN:
BLOOD OR TISSUE
VOLUME:
10 mL (blood) or 1 g (tissue)
MEASUREMENT METHOD:
INDUCTIVELY-COUPLED ARGON PLASMAATOMIC EMISSION SPECTROSCOPY (ICP-AES)
PRESERVATIVE: heparin (blood); none for tissue
ANALYTE:
elements above
SHIPMENT:
frozen for blood and "wet" tissue; routine for "dry" tissue
DIGESTION ACID:
STABILITY:
not established
CONTROLS:
collect at least 3 blood specimens from unexposed workers
3:1:1 (v/v/v) HNO 3:HClO 4:H2SO 4
FINAL SOLUTION:
10% H 2SO 4; 10 mL (blood), 5 mL (tissue)
WAVELENGTH:
varies with element; Table 2
BACKGROUND CORRECTION: spectral wavelength shift CALIBRATION:
elements in 10% H 2SO 4 or yttrium internal standard
QUALITY CONTROL:
spiked blood or tissue; reference materials
RANGE:
10 to 10,000 µg/100 g blood; 2 to 2000 µg/g tissue
ESTIMATED LOD:
1 µg/100 g blood; 0.2 µg/g tissue
PRECISION (Sr):
Table 2
ACCURACY:
Table 2
APPLICABILITY: This method is useful for monitoring the blood of workers exposed to several metals simultaneously. This is a simultaneous multielemental analysis, but is not compound-specific.
INTERFERENCES: Spectral interferences are sometimes encountered. These are minimized by judicious wavelength selection and interelement correction factors. Background corrections (spectral wavelength shift) are also made [1,2].
OTHER METHODS: This method uses a measurement technique similar to that of Methods 7300 (Elements; for air samples) and 8310 (Metals in urine).
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
