TOLUENE in Blood C7H8
MW: 92.14
METHOD: 8007, Issue 1
8007
CAS: 108-88-3
RTECS:XS5250000
EVALUATION: FULL
NIOSH and OSHA: none ACGIH: BEI 0.02 mg/L (prior to last shift of workweek) [1] DFG (German): BAT 600 µg/L (end of shift) [2] BIOLOGICAL INDICATOR OF:
Issue 1: 5 March 2013
PROPERTIES: Clear liquid; bp 110.6 °C; VP 28.4 mm Hg at 25 °C; d20 0.8669 g/mL.
Exposure to toluene
SYNONYMS: Methylbenzene, Phenylmethane, Toluol, Methylbenzol SAMPLING
MEASUREMENT
SPECIMEN: Blood (collected within 12 hours of last exposure)
TECHNIQUE:
HEADSPACE/GAS CHROMATOGRAPHY, FID
VOLUME:
ANALYTE:
Toluene
Fill a 10-mL vacuum specimen tube as completely as possible (to reduce the amount of airspace)
PRESERVATIVE:
HEADSPACE CONDITIONS:
Sodium citrate (light blue top)
SHIPMENT: Insulated container with bagged refrigerant SAMPLE STABILITY: Stable at least 30 days at 4 °C [3]
INJECTION CONDITIONS:
CONTROLS: Collect and refrigerate immediately ACCURACY RANGE STUDIED:
0.0208-5.76 µg/mL [3]
ACCURACY:
<5% [3]
BIAS:
0.0006
OVERALL PRECISION (ŜrT):
0.0157 [3]
SAMPLE STABILITY:
99.0% (Day 30) [3]
Transfer Temp,129 °C; Withdrawal, 0.80 min; Thermostat Time, 30 min; Needle, 120 °C; GC Cycle Time, 34 min; Sample Temp: 99 °C; Pressurize, 4.0 min; Inject, 0.40 min Direct injection onto column for 0.4 min, then split flow for the remainder of the run at 130 mL/min
TEMPERATURE-INJECTION: 129 °C -DETECTOR: 250 °C -COLUMN: 50 °C (hold for 2 min); 50 to 220 °C @ 10 °C/min; 220 °C (hold for 5 min) CARRIER GAS: COLUMN:
CALIBRATION:
Helium, at 21 psi head pressure Capillary, fused silica, 75-m x 0.53 mm, coated internally with 3.0 µm film 6% cyanopropylphenyl, 94% dimethylpoly-siloxane, bonded and cross-linked Analyte in control blood; with isobutanol internal standard (IS)
Estimated LOD: 0.006 µg/mL in whole blood [3] APPLICABILITY:
Can be used in monitoring the exposure of workers and drug abusers for toluene.
INTERFERENCES: None identified. The chromatographic separation conditions may be adjusted to correct separation problems. OTHER METHODS: NIOSH Method 8002 is a partially-evaluated, packed-column GC/FID method with lower accuracy and a higher detection limit. [4] The National Center for Environmental Health (NCEH)/CDC has a method for 31 volatile organic compounds in blood that includes toluene. This NCEH method is somewhat more complicated, comparable in accuracy, and is approximately 1,000 times more sensitive. [5]
NIOSH Manual of Analytical Methods (NMAM), Fifth Edition
