PENTACHLOROPHENOL in urine
C6Cl5OH
MW: 266.33
METHOD: 8303
CAS:87-86-5
8303
RTECS: SM6300000
EVALUATION: FULL
Issue 1: 15 May 1989 Issue 2: 15 August 1994
BIOLOGICAL INDICATOR OF: exposure to pentachlorophenol.
SYNONYMS: PCP; Penta.
SAMPLING
MEASUREMENT
SPECIMAN:
urine end of shift, mid to late in work week
TECHNIQUE: GAS CHROMATOGRAPHY, ECD
VOLUME:
100 mL in polyethylene bottle
ANALYTE:
pentachloroanisole (PCP methyl ether)
PRESERVATIVE: 2 to 3 drops concentrated HCl added after collection
INJECTION VOLUME:
5 µL
SHIPMENT:
ship frozen in dry ice
COLUMN:
SAMPLE STABILITY:
40 days if kept frozen
CARRIER GAS:
CONTROLS: collect 3 urine specimens from unexposed workers per study or 1 control urine per 10 samples
1.8 m x 4-mm ID glass, 4% SE-30, 6% OV-210 on 80/100 mesh silanized support 5% methane in argon, 60 mL/min
TEMPERATURE-INJECTOR: -COLUMN: -MANIFOLD: -DETECTOR: CALIBRATION:
solutions of pentachloroanisole in hexane
QUALITY CONTROL:
RANGE:
220 °C 190 °C 250 °C 300 °C
spike urine pools; corrected for creatinine content
5 to 1000 µg/L
ESTIMATED LOD: 1 µg/L [1] RECOVERY:
0.947
PRECISION (Sr):
0.03 [1]
ACCURACY:
± 10.3%
APPLICABILITY: This method measures free and conjugated PCP after hydrolysis and is useful when monitoring chronic PCP exposure via skin contact, ingestion or inhalation. Maximum urine levels are found 42 h after exposure [2]. Acute exposu res are best monitored using Method 8001 (Pentachlorophenol in blood). Urine contains about 82% free PCP and 13% PCP glucuronide [1].
INTERFERENCES: Numerous potential interferences that can be extracted from urine include chloronaphthylenes, polychlorinated biphenyls and Diuron; these are eliminated by sample cleanup.
OTHER METHODS: This method combines and replaces P&CAM 230 [3] and P&CAM 358 [1].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
