METALS in urine: METHOD 8310, Issue 2, dated 15 August 1994 - Page 5 of 5 TABLE 2. RECOVERY OF METALS FROM URINE [1,2]
Wavelength (nm)
Quantity Added, µg/50 mL sample
Precision, Sr (n = 4)
Precision, % Recovery
Accuracy ±%
Aluminum (Al)
308.2
20
0.088
100
17.2
Barium (Ba)
455.4
0.4
0.11
80
41.6 a
Cadmium (Cd)
226.5
1.0
0.12
100
23.5
Chromium (Cr)
205.6
1.0
0.078
100
15.3
Copper (Cu)
324.8
10
0.042
100
8.2
Iron (Fe)
259.9
40
0.059
100
11.6
Lead (Pb)
220.4
10
0.040
100
7.8
Manganese (Mn)
257.6
10
0.50
85
113 a
Molybdenum (Mo)
281.6
2.0
0.16
100
31.4 a
Nickel (Ni)
231.6
2.0
0.42
80
102 a
Platinum (Pt)
203.7
0.4
0.29
77
79.8 a
Silver (Ag)
328.3
2.0
0.12
100
23.5
Strontium (Sr)
421.5
4.0
0.25
100
49.0 a
Tin (Sn)
190.0
2.0
0.21
100
41.2 a
Titanium (Ti)
334.9
2.0
0.16
86
45.4 a
Zinc (Zn)
213.9
200
0.089
100
17.4
Element (Formula)
a
Does not meet the NIOSH criterion of ± 25% accuracy.
APPENDIX: POLYDITHIOCARBAMATE RESIN PREPARATION The procedure used for preparation of the polydithiocarbamate resin is that of Hackett and Siggia [5] as modified by Bary and Reilly [6]. 1. Dissolve 72 g polyethyleneimine, molecular weight 1800, in 1 L tetrahydrofuran and 28 g polymethylenepolyphenyl isocyanate in 1 L tetrahydrofuran. NOTE: Polyethyleneimine stored for one year would not dissolve in the solvent; however, fresh polyethyleneimine readily dissolved. 2. Pour these two solutions simultaneously into a large flask, allowing the two streams to mix before entering the flask. 3. Let the mixture stand at least 12 h with occasional mild agitation, then remove the solvent by filtration. 4. Wash the product twice with methanol and once with deionized water. 5. Add the product to 300 mL carbon disulfide, 100 mL ammonium hydroxide and 500 mL isopropyl alcohol; let stand 72 h. 6. Filter to remove the resin from the solvent mixture. Wash the resin three times with methanol and allow it to dry. 7. Grind and sieve the resin, saving the 60/80 mesh size for use.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
