BUTOXYACETIC ACID IN URINE C6 H12 O3
MW: 132.16
8316
CAS: 2516-93-0
RTECS: None
METHOD: 8316, Issue 1
EVALUATION: PARTIAL
Issue 1: 15 March 2003
BIOLOGICAL INDICATOR OF: Exposure to
2-butoxyethanol 2-butoxyethyl acetate
SYNONYMS:
2-butoxyethanol:
Ethylene glycol monobutyl ether, Monobutyl glycol ether, Butyl Cellosolve®, Butyl oxitol, Dowanol® EB, EGBE, Ektasolve EB®, Jeffersol EB
2-butoxyethyl acetate:
Ethylene glycol monobutyl ether acetate, Butyl Cellosolve® acetate, Butyl glycol acetate, EGBEA, Ektasolve EB® acetate,
(CAS # 111-76-2, RTECS # KJ8575000) (CAS # 112-07-2, RTECS # KJ8925000).
SAMPLING
MEASUREMENT
SPECIMEN:
Urine
TECHNIQUE:
GAS CHROMATOGRAPHY, ECD Ni-63
VOLUME:
20 mL of sample
ANALYTE:
Pentafluorobenzyl butoxyacetate, PFB-BAA
PRESERVATIVE: None SHIPMENT:
Frozen; on dry ice
SAMPLE STABILITY:
At least 9 months at -70 /C
CONTROLS:
Urine specimens from non-exposed persons, number determined by design of study.
INJECTION VOLUME: TEMPERATURE -INJECTION: -DETECTOR: -COLUMN:
5 :L
150 /C 177 /C 70 /C for 2 min, 50 /C/min to 120 /C, 2 /C/min to 170 /C
CARRIER GAS:
Helium, 10 mL/min
COLUMN:
Capillary, fused silica, 5 m x 0.53-mm ID, deactivated and uncoated, followed by 30 m x 0.53-mm ID fused silica capillary with a 2.65-µm film of polydimethyl siloxane, HP-1 or equivalent.
CALIBRATION:
Standard solutions of PFB-BAA in toluene/2-propanol
QUALITY CONTROL:
Standard solutions of butoxyacetic acid (BAA) in urine
RANGE:
10 to 450 :mol/L in urine
ESTIMATED LOD:
10 :mol/L in urine
PRECISION ( þ r ):
0.13
ACCURACY:
Not determined
APPLICABILITY: Urinary butoxyacetic acid (BAA) is a biomarker of exposure to 2-butoxyethanol and 2-butoxyethyl acetate. Both 2-butoxyethanol and 2-butoxyethyl acetate are metabolized to butoxyacetic acid (BAA) and N-butoxyacetylglutamine, which are excreted in urine [1]. Since BAA produces the adverse hematogic effects attributed to exposures to 2-butoxyethanol and 2-butoxyethyl acetate, urinary BAA serves also as a biomarker to these particular exposure-related adverse health effects [2]. INTERFERENCES: No analytical interferences found. Consumption of ethanol is predicted to inhibit metabolism of 2-butoxyethanol to BAA [2], and thus may effect the accuracy of biomonitoring. OTHER METHODS: This method is based on those of Smallwood et al. [3] and Johanson [4]. Grosenken et al. [5] published a method using lyophilization, derivatization with pentafluorobenzyl (PFB) bromide, then GC. The method of Rettenmeier et al. [1] determined both free BAA and its conjugate with glutamine using extraction and derivatization with 4-nitrobenzyl bromide, then HPLC. Sakai et al. [6] used acid hydrolysis, extraction, derivatization with trimethylsilyldiazomethane, then GC to determine free plus conjugated BAA.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition
