3-BROMOPROPIONIC ACID in URINE: METHOD 8324, Issue 1, dated 29 August 2014 - Page 9 of 9
TABLE 1. Recovery studies of 3-bromopropionic acid:
(A) multilevel recovery study of 3-BPA from spiked urine samples1, (B) recovery of 20 μg/mL 3-BPA spikes from individual urines of 20 non-exposed volunteers2 Note: Overall recovery of all samples was 95% and overall RSD was 3.1% (n = 47.)
(A) Spike level (μg/mL)
Mean 3-BPA recovered, (n = 9, μg/mL)
Average % Recovery
Standard Deviation (μg/mL)
% Relative Standard Deviation
2
1.91
96
0.11
5.7
10
9.32
93
0.13
1.4
50
48.9
98
0.36
0.7
(B) Volunteer urine Spike level (μg/mL) 20
Mean 3-BPA recovered (n = 20, μg/mL)
Average % Recovery
19.0
95
Standard Deviation (μg/ mL)
% Relative Standard Deviation
0.48
2.5
1 Three different spiked urine samples were prepared at each level and chromatographed on three separate experimental trial runs (a total of nine samples at each spike level were analyzed.) 2 All non-spiked specimens showed no 3-BPA derivative peak in the chromatograms.
NIOSH Manual of Analytical Methods (NMAM), Fifth Edition
