S-Benzylmercapturic acid and S-phenylmercapturic acid in urine: METHOD 8326, Issue 1, dated 20 May 2014 - Page 6 of 10
individual deuterated internal standards, the use of amber glass autosampler vials or other means of reducing light exposure is recommended to ensure sample stability during extended chromatographic batch runs. Range. This method should be considered accurate for the estimation of BMA and PMA in human urine within the 0.5 to 50 ng/mL standard curve range. Field samples at higher levels can be diluted to a concentration within that range for analysis. REFERENCES: [1] ACGIH [2013]. TLVs and BEIs based on the documentation of the Threshold Limit Values for chemical substances and physical agents and Biological Exposure Indices. Cincinnati, Ohio: American Conference of Governmental Industrial Hygienists. www.acgih.org [Date accessed: April 2014.] [2] Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area [2013]. List of MAK and BAT values 2013; Maximum Concentrations and Biological Tolerance Values at the Workplace. DFG: Deutsche Forschungsgemeinschaft, Wiley-VCH Verlag GmbH & Co. http:// www.dfg.de/en/dfg_profile/publications/senate_commissions/index.html#micro7535782 [Date accessed April 2014.] [3] SUVA (Swiss National Accident Insurance Fund) [2013]. Protection de la santé au poste de travail. Valeurs limtes d’exposition aux postes de travail. http://www.suva.ch/english/startseite-en-suva/ praevention-en-suva/publications-en-suva.htm [Date accessed: April 2014.] [4] Institut für Arbeitsschutz der Deutschen Gesetzlichen Unfallversicherung (IFA) http://www.dguv. de/ifa/Gefahrstoffdatenbanken/GESTIS-Internationale-Grenzwerte-für-chemische-Substanzenlimit-values-for-chemical-agents/index-2.jsp [Date accessed: April 2014.] [5] Boogaard PJ, Van Sittert NJ [1995]. Biological monitoring of exposure to benzene: A comparison between S-phenylmercapturic acid, trans, trans-muconic acid and phenol. Occup Environ Med 52:611-620. [6] Schettgen T, Musiol A, Kraus T [2008]. Fast determination of urinary S-phenylmercapturic acid and S-benzylmercapturic acid by column-switching liquid chromatography-mass spectrometry. J Chromatogr B 863:283-292. [7] FDA [2001]. Guidance for industry: Bioanalytical method validation, U.S. Food and Drug Administration, Rockville, MD. [1]. [Date accessed April 2014.] [8] Lovreglio P, Barbieri A, Carrieri M, Sabatini L, Fracasso ME, Doria D, Drago I, Basso A, D’Errico MN, Bartolucci GB, Violante FS, Soleo L [2010]. Validity of new biomarkers of internal dose for use in the biological monitoring of occupational and environmental exposure to low concentrations of benzene and toluene. Int Arch Occup Environ Health 83:341-356. [9] B’Hymer C [2011]. Backup Data Report, Method 8326. [10] B’Hymer C [2011]. Validation of an HPLC-MS/MS method for the determination of urinary S-benzylmercapturic acid and S-phenylmercapturic acid. J Chromatogr Sci 49:547-553. [11] Alwis KU, Blount BC, Britt AS, Patel D, Ashley DL [2012]. Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MS-MS). Anal Chim Acta 750:152-160. [12] CDC [2007]. 2007 Guidelines for isolation precautions: Preventing transmission of infectious agents in healthcare settings. [2]. [Date accessed: April 2014.]
NIOSH Manual of Analytical Methods (NMAM), Fifth Edition
