CHLORINATED AND ORGANONITROGEN HERBICIDES (HAND WASH): METHOD 9200 - Page 6 of 11
Parameter
Conditions
Column Parameters: Stationary phase(2)
DB-1
DB-5
DB-5ms
DB-17(3)
DB-1701(4) DB-210(4)
DB-225(4)
Length (meters)
30
30
30
30
30
DB-WAX
30
30
30
I.D. (millimeters)
0.25
0.32
0.32
0.25
0.53
0.32
0.32
0.32
Film thickness (µm)
0.25
0.50
1.00
0.25
1.00
0.25
0.25
0.50
120
50
90
90
90
140
140
160
0
1
1
1
0.5
0
0
0
5
10
35
35
15
3
5
5
160
160
180
Oven Temperatures: Initial temperature (oC) Initial temperature hold time (min.) o
First temperature ramp ( C/min.) o
First intermediate temperature ( C) o
Second temperature ramp ( C/min.) Second intermediate temperature (oC) Third temperature ramp (oC/min.) o
Final temperature ( C)
5
5
2
200
200
210
3
3
10
250
290
230
230
235
215
220
250
4
5
9
9
10
5
15
20
Helium
Helium
Helium
Helium
Helium
Helium
Helium
Helium
Head pressure (p.s.i.)
10
10
12
12
3.5
10
10
10
Injection volume (µL)
2-4
2-4
2-4
2-4
2
2-4
2-4
2-4
splitless
splitless
splitless
splitless
splitless
splitless
splitless
splitless
Final temperature hold time (min.) Mobile Phase and Injection conditions: Carrier Gas
Injection mode
(1) Actual column and conditions may vary depending on analyte, interferences, and analytical objectives. The conditions given above correspond to Table 4. (2) Other types of fused silica capillary columns may also work well. (3) Column and conditions used for method evaluation. Good column for separation of atrazine and simazine. (4) Useful columns for separating cyanazine from other listed analytes.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition
