in the same yeast a sort of servant given by Nature to effect this digestion. The servant is the unorganized inversive ferment. This ferment is soluble, and, as it is not a plant, but an unorganized body destitute of sensibility, it has not gone to sleep under the action of the ether, and thus continues to fulfill its task."
In the experiment already recorded on the germination of seeds the interest is by no means confined to that which attaches itself to the arrest of the organizing functions of the seed, those namely which manifest themselves in the development of the radicle, and plumule, and other organs of the young plant. Another phenomenon of great significance becomes at the same time apparent: the anæsthetic exerts no action on the concomitant chemical phenomena which in germinating seeds show themselves in the transformation of starch into sugar under the influence of diastase (a soluble and non-living ferment which also exists in the seed), and the absorption of oxygen with the exhalation of carbonic acid. These go on as usual, the anæsthesiated seed continuing to respire, as proved by the accumulation of carbonic acid in the surrounding air. The presence of the carbonic acid was rendered evident by placing in the same vessel with the seeds which were the object of the experiment a solution of barytes, when the carbonate became precipitated from the solution in quantity equal to that produced in a similar experiment with seeds germinating in unetherized air.
So, also, in the experiment which proves that the faculty possessed by the chlorophyllian cells of absorbing carbonic acid and exhaling oxygen under the influence of light may be arrested by anæsthetics, it could be seen that the plant, while in a state of anæsthesia, continued to respire in the manner of animals; that is, it continued to absorb oxygen and exhale carbonic acid. This is the true respiratory function which was previously masked by the predominant function of assimilation, which devolves on the green cells of plants, and which manifests itself under the influence of light in the absorption of carbonic acid and the exhalation of oxygen.
It must not, however, be supposed that the respiration of plants is entirely independent of life. The conditions which bring the oxygen of the air and the combustible matter of the respiring plant into such relations as may allow them to act on one another are still under its control, and we must conclude that in Claude Bernard's experiment the anæsthesia had not been carried so far as to arrest such properties of the living tissues as are needed for this.
The quite recent researches of Schützenberger, who has investigated the process of respiration as it takes place in the cell of the yeast-fungus, have shown that vitality is a factor in this process. He has shown that fresh yeast, placed in water, breathes like an aquatic animal, disengaging carbonic acid, and causing the oxygen contained in the water to disappear. That this phenomenon is a function of the
