only in two or three out of a hundred cases where the flasks had been sealed at the top of the mountain, and to a proportionate extent in those sealed at the intermediate elevations. What, now, did these experiments prove? Simply this, that the development of these organic forms was in direct proportion to the number of germs in the air. It did not settle the question of spontaneous generation, but it showed that false conclusions had been deduced from the experiments which had been cited to prove it.
A still more striking illustration of the same method of questioning Nature is to be found in the investigation of Sir Humphry Davy on the composition of water. The voltaic battery which works our telegraphs was invented by Volta in 1800; and later, during the same year, it was discovered in London, by Nicholson and Carlisle, that this remarkable instrument had the power of decomposing water. These physicists at once recognized that the chief products of the action of the battery on water were hydrogen and oxygen gases, thus confirming the results of Cavendish, who in 1781 had obtained water by combining these elementary substances; oxygen having been previously discovered in 1775, and hydrogen at least as early as 1766. It was, however, very soon also observed that there were always formed by the action of the battery on water, besides these aëriform products, an alkali and an acid, the alkali collecting around the negative pole and the acid around the positive pole of the electrical combination. In regard to the nature of this acid and alkali there was the greatest difference of opinion among the early experimenters on this subject. Cruickshanks supposed that the acid was nitrous acid, and the alkali ammonia. Desormes, a French chemist, attempted to prove that the acid was muriatic acid; while Brugnatelli asserted that a new and peculiar acid was formed, which he called the electric acid.
It was in this state of the question that Sir Humphry Davy began his investigation. From the analogies of chemical science, as well as from the previous experiments of Cavendish and Lavoisier, he was persuaded that water consisted solely of oxygen and hydrogen gases, and that the acid and alkali were merely adventitious products. This opinion was undoubtedly well-founded; but, great disciple of Bacon as he was, Davy felt that his opinion was worth nothing unless substantiated by experimental evidence, and accordingly he set himself to work to obtain the required proof.
In Davy's first experiments the two glass tubes which he used to contain the water were connected together by an animal membrane, and he found, on immersing the poles of his battery in their respective tubes, that, besides the now well-known gases, there were really formed muriatic acid in one tube and a fixed alkali in the other. Davy at once, however, suspected that the acid and alkali came from common salt contained in the animal membrane, and he therefore rejected this material and connected the glass tubes by carefully-washed cotton
