Page:Wound infections and some new methods for the study of the various factors which come into consideration in their treatment.djvu/25

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BIOLOGICAL EVOLUTION

11

into the flame of our Bunsen, and let gravity bend it round into a siphoning curve.

Taking one of these capillary cultivation tubes we bring down the siphon end upon our drop of serum (fig. 5, A), and let this run in, afterwards tilting the tube so as to take in, as a rear-guard, a good-sized

FIG. 5.

Method for making anaerobic cultures in capillary tubes.

dividing bubble of air. This done, we fill into our tube, by siphonage, from watch-glasses placed conveniently to hand, first a small quantity of pyrogallic acid, and then, without dividing off, a small quantity of caustic soda (fig. 5, B and C). We now further incline the tube, and let the contained fluids